Fig. 3.

AMPK levels following stimulation differ in Triton X-100 (TX100) vs. SDS solubilized acinar cell lysates. Acinar cells were unstimulated (CTL) or treated with cerulein (CER, 100 nM) for 60 min. Samples were solubilized in 1% Triton X-100 or 1.7% SDS (1X Laemmli buffer), and subsequent immunoblots were probed with antibodies specific to AMPK-α (A) or AMPK-β (B). A significant decrease of AMPK levels is observed in TX100 extracts but not in SDS lysates. Top: representative blots. Bottom: densitometry based on 3 to 5 experiments. CTL, open bars; CER, solid bars. *P < 0.05 vs. CTL; n ≥ 3.