Fig. 6.

Zymogen activation, in response to cerulein hyperstimulation, occurs in heavy (zymogen granule-enriched) fractions. Following induction of cerulein pancreatitis in vivo for 60 min, pancreatic fractions were prepared from cerulein (CER)-treated or untreated controls (CTL) rat pancreas using differential centrifugation (see materials and methods). Normalized (RFU/s/mg protein) levels of trypsin (A) and chymotrypsin (B) activity in subcellular pancreatic fractions. The highest levels of trypsin and chymotrypsin activity were observed in the heaviest fractions (zymogen granule-enriched, P1.8, P3) from CER-treated animals. *P < 0.05 vs. CTL; n ≥ 3.