Fig. 3.

Conditional Men1 deletion in the duodenum does not alter G cell number. A and B: gastrin immunostaining in duodenum of 12-mo-old WT and VC:Men1^FL/FL mice. Original magnification: ×200. C: G cell quantification in duodenum of 12-mo-old VC:Men1^FL/FL and WT mice. Values are numbers of gastrin-immunostained cells per high-power field (HPF). D–G: coimmunostaining of paraffin sections from duodenum for gastrin (green) and menin (red) with DAPI nuclear stain (blue) to identify double-positive cells in WT and VC:Men1^FL/FL mice within crypt (D and F) and villi (E and G). Original magnification: ×600.