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Menin deletion in the antrum of VC:Men1FL/FL mice. A: RT-qPCR analysis of Men1 mRNA in the stomach of 3-, 6-, and 12-mo-old WT or VC:Men1FL/FL mice. Values are means ± SE; n = 3–4. *P < 0.05. B: Western blot analysis of menin protein in antrum of 2 WT (lanes 1 and 2) and 2 VC:Men1FL/FL (lanes 3 and 4) mice at 12 mo of age. C and D: menin (red) immunofluorescence in the antrum of 12-mo-old WT and VC:Men1FL/FL mice. Blue, DAPI. Original magnification: ×400.
