Fig. 3.

DMNV neuron response to nesfatin-1. A: representative trace of a single DMNV neuron response to 10⁻⁶ M nesfatin-1. Exposure to nesfatin-1 for 300 s caused an increment in intracellular Ca²⁺ concentration ([Ca²⁺]_i) that peaked within 30 s at >100% over baseline, maintained a plateau for the duration of nesfatin-1 exposure, and subsequently returned to baseline. B and C: dose-dependent relationship between nesfatin-1 and Ca²⁺ response, expressed as maximal average change in fluorescence ratio (ΔF/F₀, where ΔF is change in fluorescence ratio and F₀ is basal fluorescence ratio) and percentage of cells responding (n = 120, 71, 71, 79, and 102, respectively). *P < 0.05 vs. the next-lower concentration.