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Representative trace from a single cell showing that inhibition of nesfatin-1 induced Ca2+ transient by NiCl2. DMNV neurons were superfused with nesfatin-1 for 30 s and allowed to return to baseline fluorescence ratio. Cells were pretreated with 3 mM NiCl2 for 180 s and then with nesfatin-1 in the presence of NiCl2 for 30 s. NiCl2, a nonspecific Ca2+ channel blocker, abolished nesfatin-1-induced intracellular Ca2+ transients.
