Fig. 1.

Rottlerin inhibits cAMP-induced PKCδ translocation to the plasma membrane. HuH-NTCP cells were treated with or without 5 μM rottlerin for 30 min, followed by treatment with or without 100 μM 8-chlorophenylthio cAMP (CPT-cAMP) for 15 min. PKCδ translocation was measured by a biotinylation method. Top: representative immunoblot of the amount of PKCδ in the plasma membrane (PM). Bottom: densitometric analysis is shown. E-cadherin (E-cad) was used as a loading control. The relative values of PKCδ in the PM are expressed as means ± SE (n = 4). Data were analyzed by paired t-test. *Significantly different (P < 0.05) from control values in the absence of cAMP and rottlerin; #significantly different (P < 0.05) from cAMP treatment.