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. 2012 Jun 28;303(5):G657–G665. doi: 10.1152/ajpgi.00529.2011

Fig. 10.

Fig. 10.

DN-PKCδ and siRNA PKCδ inhibited cAMP-induced MRP2 translocation. HuH-NTCP cells were transfected with EV and DN-PKCδ (A), or transfected with scrambled siRNA (control) and siRNA PKCδ (B) followed by treatment with or without 100 μM CPT-cAMP for 15 min. A biotinylation method was used to determine PM MRP2. A: representative immunoblot of MRP2 and E-cadherin (top); densitometric analysis (bottom). Relative values of MRP2 in the PM are expressed as means ± SE (n = 3). Data were analyzed by 1-way ANOVA. B: representative blot of PM MRP2 and E-cadherin (top); densitometric analysis (bottom). Relative values of MRP2 in the PM are expressed as means ± SE (n = 3). Data were analyzed by paired t-test. *Significantly different (P < 0.05) from control values in the absence of cAMP; #significantly different (P < 0.05) from control values in the presence of cAMP.