Fig. 3.

Rottlerin does not inhibit cAMP-induced Akt phosphorylation. HuH-NTCP cells were treated with or without 5 μM rottlerin for 30 min, followed by treatment with or without 100 μM CPT-cAMP for 10 min. Cell lysates from treated cells were subjected to immunoblot analysis. A representative immunoblot is shown at top with the densitometric analysis shown at bottom. Akt activity was determined from the ratio of phosphorylated Akt to total Akt and is expressed as means ± SE (n = 3). Data were analyzed by 1-way ANOVA. *Significantly different (P < 0.05) from control values in the absence of cAMP and rottlerin.