Fig. 6.

Small interfering RNA (siRNA) PKCδ increased PM PKCδ. A: level of PKCδ protein was downregulated up to 80% in cells transfected with siRNA PKCδ for 48 h compared with cells transfected with scrambled siRNA (Con) or nontransfected cells (Non-trans) B: HuH-NTCP cells were transfected with scrambled siRNA and siRNA PKCδ, followed by treatment with or without 100 μM CPT-cAMP for 15 min. A biotinylation method was used to determine PM PKCδ. Representative immunoblots of PM PKCδ (top), total PKCδ (middle), and the densitometric analysis (bottom) are shown. The relative values of PKCδ in the PM are expressed as means ± SE (n = 4). Data were analyzed by paired t-test. *Significantly different (P < 0.05) from control values in the absence of cAMP.