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. 2012 Jun 28;303(5):G657–G665. doi: 10.1152/ajpgi.00529.2011

Fig. 7.

Fig. 7.

siRNA PKCδ increased Rab4 activity and the amount of PM NTCP. HuH-NTCP cells were transfected with scrambled siRNA (control) or siRNA PKCδ, followed by treatment with or without 100 μM CPT-cAMP for 15 min and then determination of Rab4 activity (A). A biotinylation method was used to determine PM NTCP (B). A: representative blot of Rab4-GTP and total Rab4 (top); densitometric analysis (bottom). Rab4 activity (means ± SE, n = 3) was expressed as a ratio of Rab4-GTP to total Rab4. Data were analyzed by 1-way ANOVA. *Significantly (P < 0.05) different from control values in the absence of cAMP in cells transfected with scrambled siRNA. Control values in the absence and presence of cAMP are #significantly different (P < 0.05) by paired t-test. B: representative immunoblot of PM NTCP and E-cadherin (top); densitometric analysis (bottom). Relative values of NTCP in the PM are expressed as means ± SE (n = 3). Data were analyzed by 1-way ANOVA. *Significantly (P < 0.01) different from control values in the absence of cAMP. Control values in the absence and presence of cAMP are #significantly different (P < 0.05) by paired t-test.