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. 2012 Jun 28;303(5):G657–G665. doi: 10.1152/ajpgi.00529.2011

Fig. 8.

Fig. 8.

LY294002 inhibited cAMP-induced PKCδ translocation to the PM. HuH-NTCP cells were treated with or without 20 μM LY294002 for 30 min, followed by treatment with or without 100 μM CPT-cAMP for 15 min. PKCδ translocation was measured by a biotinylation method. Top: representative immunoblot of the amount of PKCδ in the PM. Bottom: densitometric analysis is shown. E-cadherin was used as a loading control. The relative values of PKCδ in the PM are expressed as means ± SE (n = 3). Data were analyzed by 1-way ANOVA. *Significantly (P < 0.05) different from control values in the absence of cAMP and LY294002; #significantly (P < 0.05) different from cAMP treatment.