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. 2012 Jul 12;303(5):G578–G588. doi: 10.1152/ajpgi.00234.2012

Fig. 4.

Fig. 4.

Regulation of DHRS3, β-carotene 15,15′-monooxygenase 1 (BCMO1), and lecithin:retinol acyltransferase (LRAT) mRNA expression in liver of adult rats treated in vivo with RA, LPS, or both. A: schema of pathway involving genes metabolizing retinal. RE, retinyl ester; REH, RE hydrolase; RDH, retinol dehydrogenase; RALDH, retinaldehyde dehydrogenase. B, D, and F: total RNA was extracted from individual liver samples (n = 6/group) and then measured by real-time quantitative RT-PCR for DHRS3, BCMO1, or LRAT, as well as for 18S RNA as the control. B: DHRS3 expression in liver of adult rats fed vitamin A-adequate diet and treated with RA (500 μg/kg body wt), LPS (50 μg/kg body wt) (45), or both for 6 h. C: effect of actinomycin D (Act D) on expression of DHRS3 mRNA in liver of adult rats. Rats were treated with actinomycin D alone or with RA for 6 h, and liver tissues were collected for total RNA extraction and analysis. Values are means ± SE (n = 3/group). D: BCMO1 mRNA from samples used in B. E: Northern blot analysis of LRAT mRNA from liver of rats treated as described in B. ATRA, all-trans-RA. F: quantitative RT-PCR for LRAT mRNA from samples used in B. Different letters indicate significant difference: P < 0.05 for B, D, and F; P < 0.0001 for C.