Fig. 10.

Real-time PCR and ELISA for macrophage inflammatory protein 1α (MIP-1α). HET-1A cells were treated with the selected HCl exposure protocol and then used to determine MIP-1α RNA expression by real-time PCR (left) and protein expression by ELISA (middle). Repeated exposures to pH 5 over 48 h caused a significant increase in mRNA and protein expression for MIP-1α (*P < 0.05). Relative mRNA expression was calculated with respect to mRNA from untreated cells. Values are means ± SE; n = 3 for 3 different sets of data from 3 separate experiments. Right: real-time PCR for human biopsies from EE patients and normal controls. Increased MIP-1α mRNA in EE patients parallels increase in HET-1A cells after exposure to HCl.