Mouse ES cells were plated at low density in the ES cell medium containing serum and LIF, and then transferred to N2B27 without LIF after overnight incubation (Day 0). (A) Co-staining patterns of Zfp568 with the ES cell markers Oct4 and Sox2, respectively, of ES cells in the ES medium. (B) Co-staining patterns of Zfp568 with the neural stem cell marker Nestin and neuronal markers, Tuj1 and MAP2, on different days in the N2B27 medium. The percentages of single- and double- stainings, as listed under each panel, were each calculated by scoring the cells in at least three random chosen fields from two independent sets of experiments. (n = 10 for Day3, n = 7 for Day 7, n = 7 for Day 9). Bar, 50 µm.