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. 2012 Oct 10;7(10):e47496. doi: 10.1371/journal.pone.0047496

Figure 6. Adipogenic differentiation of cloned low adipogenic cells after Zfp423 transfection.

Figure 6

A) adipogenic gene expression in control (eGFP transfected low adipogenic cells), Zfp423 transfected low adipogenic cells, and cells without transfection. B) Transmissive scanning of Oil-Red-O stained cells. C) Quantification of Oil-Red-O staining by absorbance measurement at 520 nm. D) 100× magnified image of high adipogenic cells. E) 100× magnified image of low adipogenic cells. F) 100× magnified image of Zfp423 transfected low adipogenic cells. G) 100× magnified image of eGFP tranfected low adipogenic cells. (abMeans bearing different superscripts indicate significant difference, P<0.05, Mean ± SE; n = 3).