Figure 9. Effect of purified recombinant HMGB1 on IL-6 secretion by macrophages.

HeNC2 cells were stimulated with various amount of HMGB1 with LTA (solid line, 1 µg/ml)) or without (dashed line). IL-6 concentrations were determined by ELISA. *P<0.02 Activation with LTA plus 100 ng/ml HMGB1 compared to activation with LTA alone. These results denote mean +/− standard error (n = 3) and are representative of three independent experiments.