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. 2012 Oct 10;7(10):e46494. doi: 10.1371/journal.pone.0046494

Figure 5. i-SUB5-6 is present in Chlamydomonas I1 mutants and N-DRC mutants.

Figure 5

(A–H) A comparison of tomographic slices from averages of the distal part of DMT1 from Chlamydomonas pWT (A and B), I1-dynein-lacking mutant pf9-3 (C and D), a combined average of the N-DRC mutants sup-pf-3 and pf2 (E and F), and the dynein-IA4-lacking mutant ida6 (G–I) indicates that the lack of I1 dynein or IA4 (dynein e) or a dramatic reduction in the N-DRC has little effect on the i-SUB5-6 structure (orange arrowheads). The tomographic slices were viewed from the front (left column) and the bottom (right column) and show the same locations of the axonemal repeat as shown in Figure 3E and 3G, respectively. The orientation of the tomographic slice shown in (B) is indicated by red dashed lines in (A). (I and J) Tomographic slices from combined averages of DMT2-9 show the absence of IA4 in the ida6 axoneme (I) compared to pWT (J). Scale bar (B): 10 nm.