Table 1. Chlamydomonas strains used in this study and presence of discussed features.

			Number of Tomograms
Strain	Swimming	Reference	analyzed	i-SUB5-6	proximal 1–2 bridge
	phenotype			present	present	absent
WT (CC-125, 137c)	WT waveform, fast swimming	51, 77	19	19	5	14
pWT (pf2-4::PF2-GFP a, ida6::IDA6-GFP b, ida6::IDA6-SNAP c)	WT waveform, fast swimming	34, 37	11	11	3	8
I1 mutant (pf9-3, CC-3913)	Slow swimming	51, 55	7	7	1	6
N-DRC mutants (sup-pf-3, CC-1399, pf2)	Abnormal waveform, slow swimming	36, 37	8	8	2	6
N-DRC mutant also lacking IA4(ida6, CC-3090)	Abnormal waveform, slow swimming	78	6	6	2	4

Some of the tomograms from these strains were previously used with different image processing methods to analyze other axonemal complexes [30], [37], [51].

^aStrain provided by Raqual Bower and Mary E. Porter (University of Minnesota). The N-DRC defective pf2 mutant strain [36] was rescued with a GFP-tagged WT PF2 gene. The resultant strain, pf2-4::PF2-GFP, is structurally and phenotypically indistinguishable from WT.

^bStrain provided by Douglas Tritschler and Mary E. Porter (University of Minnesota). The N-DRC defective ida6 mutant strain [78] was rescued with a GFP-tagged WT IDA6 gene and the resulting ida6::IDA6-GFP strain has the same motility and structure as WT.

^cStrain provided by Douglas Tritschler and Mary E. Porter (University of Minnesota). The N-DRC defective ida6 mutant strain [78] was rescued with a SNAP-tagged WT IDA6 gene. The resultant strain, ida6::IDA6-SNAP, is structurally and phenotypically indistinguishable from WT.