Table 2. Correlation of DMT numbering systems and doublet-specific features.
Reference | Doublet number | ||||||||||
DMT numbering system | Unified numbering | this study | D-I | D-II | D-III | D-IV | D-V | D-VI | D-VII | D-VIII | D-IX |
Sea urchin ( S.p. ) | 7 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | |
Chlamydomonas ( C.r. ) | 15 | 6 | 7 | 8 | 9 | 1 | 2 | 3 | 4 | 5 | |
Doublet-specific features | C.r. & S.p. i-SUB5-6, S.p. o-SUB5-6, C.r. 1–2 bridge | this study; 7, 15 | − | − | − | − | − | − | − | − | − |
S.p. dynein IA2,3,4 | this study | + | + | + | + | − | + | + | + | + | |
C.r. dynein IA3,4 | this study; 16 | + | + | + | + | − | + | + | + | + | |
S.p. dynein IAX | this study; | − | + | + | + | − | + | + | + | − | |
C.r. dynein IAX | this study; 16a | + | + | + | − | − | + | + | + | − | |
C.r. beak | 42 | + | − | − | − | + | − | − | − | + | |
C.r. I1 dynein modification | 34b | − | − | − | − | − | − | + | + | − | |
S.p. RS joist | 13 | − | − | + | + | − | − | + | + | + | |
S.p. RS spur | 13 | − | + | − | − | − | − | − | − | − |
Bui et al. [16] reported that in Chlamydomonas IAX (dynein b/g) is clearly missing from DMT9, and maybe missing from DMT1– consistent with the data presented here; however, the authors did not report that IAX is greatly reduced at DMT5, as shown here.
Heuser et al. [34]: in Chlamydomonas axonemes the intermediate chain and light chain complex (ICLC) of the I1 dynein contains a doublet-specific protrusion only found on DMTs 3 and 4, but not on the other seven DMT.