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. 2012 Oct 10;7(10):e46956. doi: 10.1371/journal.pone.0046956

Figure 7. Effect of PGs on the proliferation of stimulated PBMC in presence or absence of CMs obtained from hAMTC or hAM treated or not with indometahcin.

Figure 7

(A) Lymphocyte proliferation, stimulated with anti-CD3, in presence of different amount of prostaglandin (PG) PGD2 (⧫), PGE2 (□), PGF2α (Δ) or PGI2 (X). (B) Lymphocyte proliferation, stimulated with anti-CD3, in presence of 100 μl of control medium (UltraCulture) or CM derived from untreated (CM-hAMTC) or indomethacin treated (CM-hAMTC Indo) hAMTC with (white bars) or without (black bars) the addition of a mix of PGs (PGE2 500 ng/ml + PGD2 100 ng/ml + PGF2α 25 ng/ml). (C) Lymphocyte proliferation, stimulated with anti-CD3, in presence of 100 μl of control medium (UltraCulture) or CM derived from untreated (CM-hAM) or indomethacin treated (CM-hAM Indo) hAM with (white bars) or without (black bars) the addition of a mix of PGs (PGE2 25 ng/ml + PGD2 6 ng/ml + PGF2α 10 ng/ml). * = p<0.05, ** = p<0.01, *** = p<0.001.