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. 2012 Oct 10;7(10):e46601. doi: 10.1371/journal.pone.0046601

Figure 1. Relative luciferase activity of the constructs containing the putative SPRN promoter.

Figure 1

(A) Schematic representation of the bovine SPRN fragments used in this study. The promoter activity of two constructs (containing fragments g.808–1691 and g.805–2129, respectively) was assessed. The two boxes are indicated as exon 1 and 2 of the bovine SPRN gene spanning from g.1355 to 1465 and from g.2192 to 2790, respectively. (B) Relative luciferase activity of the cattle or buffalo SPRN promoter in N2a cells. The data show the constructed plasmid activity after normalization with the co-transfected reference vector (pRL-TK), and relative to the activity of pGL3-Basic vector, which the activity was set to 1. Results are shown as mean ± SEM of three independent experiments. Each independent experiment was performed with three separately transfected wells. * Indicates significant differences for relative luciferase activity between the constructs (g.805–2129) in cattle and buffalo.