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. 2012 Oct 10;7(10):e46387. doi: 10.1371/journal.pone.0046387

Figure 1. Expression of Sox21 during chicken inner ear development.

Figure 1

(A) Sox21 mRNA showed faint levels of expression at E3 in the otic vesicle. (B) Upregulation of Sox21 expression was observed in the presumptive posterior crista and the presumptive superior crista regions at E5. (C) At E7 Sox21 transcripts were detected in the basilar papilla, the utricle, the saccule and the posterior, superior and lateral cristae. (C’) in the utricle, Sox21 is strongly expressed in the striola region (entire utricle is marked with black dotted line). (C’’) Transverse cryosection through the basilar papilla reveals Sox21 transcripts are restricted to the support cell layer. (C’’’) Transverse cryosection through the utricle reveals that Sox21 transcripts are enriched in the hair cell layer. (D) At E15 Sox21 transcripts were still present in the basilar papilla, the utricle, the saccule and the posterior, superior and lateral cristae. D’) Transverse cryosection through the E15 basilar papilla reveals Sox21 transcripts remain restricted to the support cell layer. (D’’) Transverse cryosection through the E15 utricle reveals Sox21 transcripts are enriched in the hair cell layer. (D’’’) Transverse cryosection through the E15 cristae reveal Sox21 transcripts are enriched in the hair cell layer. Picture shown is of a lateral crista; all cristae exhibited the same expression pattern. ov: otic vesicle; sc: superior crista; pc: posterior crista; lc: lateral crista; ut: utricle; bp: basilar papilla; HC: hair cell layer; SC: support cell layer.