Fig. 3.

Altered EMT marker expression in residual tumour fraction after TA treatment. Mice with colorectal liver metastases had two selected tumours thermally ablated. a and b Serial sections of formalin-fixed control tissues stained with antibodies against E-cadherin and Zeb1, respectively. c and d Serial sections of formalin fixed thermally ablated tumour tissues stained with antibodies to E- cadherin and Zeb1 respectively. N = tumour necrotic area, TN = thermally induced necrotic area, T = tumour, L = liver. Scale bar 250 μm, insert scale bar 50 μm