Fig. 6.

ROS production by neutrophils in the presence of hrHspA1A. a Neutrophils were pre-incubated with exogenous HspA1A (hrHspA1A, 5 and 10 ng/ml) for 30 min and then stimulated with PMA or fMLP. b Neutrophils were pre-treated or not with IRAK1/4 inhibitor (10 μM) for 30 min, then incubated with hrHspA1A (10 ng/ml) for 30 min, and stimulated with PMA or fMLP. ROS production was assessed using the luminol-enhanced CL assay. Data are expressed as mean values of relative light unit total (RLU total) ± SD, n = 6. *p ≤ 0.05, neutrophils versus neutrophils + hrHspA1A, Wilcoxon’s signed-rank test