Figure 3. Isolation of CD163⁺ macrophages and cytokine expression.

(A) Uterine macrophages were isolated by CD163 positive selection. The purity of the isolated cells typically ranged between 89-95 percent, as determined by staining for CD163 compared to unstained controls. (B) CD163⁺ selected macrophages from 3 individual donors were stimulated with 10 ng/ml LPS for 24 hours and analyzed for cytokine secretion in culture supernatants by Bio-Plex analysis.