Fig. 2.

Mutation of putative promoter elements does not effect the activation of CHS2 and CHS8 in response to cell wall stresses. a C. albicans strains containing a single copy of the reporter constructs with the wild type CHS2 and CHS8 promoters and those with mutated RLM1-elements, CDREs, and ATF/CREB-elements were assayed for β-galactosidase activity following growth from an OD₆₀₀ of 0.1–1.0 in the presence and absence of 100 μg/ml CFW, 100 mM CaCl₂ and 1 M sorbitol. Each measurement is the average β-galactosidase activity measured from three independent transformants for each reporter construct assayed in triplicate ± SD (n = 9). b Diagrams representing the reporter constructs containing the individual mutations of the putative promoter elements and summary of the average fold-induction ± the standard deviation for each of the reporter constructs shown above