Table 6.
The PKC cell wall integrity-, Ca2+/calcineurin- and the HOG pathway act on a minimal CaCHS2 promoter
| Reporter | Strain | Fold-induction with CaCl2 and CFWa | p-value |
|---|---|---|---|
| pCHS2plac-347 | CAI-4 | 7.88 ± 0.69 | |
| pCHS2plac-347 | crz1Δ | 7.46 ± 0.47 | |
| pCHS2plac-347 | cna1Δ | 4.33 ± 0.66 | 0.003 |
| pCHS2plac-347 | mkc1Δ | 3.07 ± 0.35 | 1.25 × 10−4 |
| pCHS2plac-347 | hog1Δ | 2.49 ± 0.41 | 3.36 × 10−5 |
| pCHS8plac-125 | CAI-4 | 10.69 ± 0.73 | |
| pCHS8plac-125 | crz1Δ | 7.02 ± 0.65 | |
| pCHS8plac-125 | cna1Δ | 16.27 ± 1.84 | |
| pCHS8plac-125 | mkc1Δ | 26.81 ± 2.88 | 1.06 × 10−4 |
| pCHS8plac-125 | hog1Δ | 16.69 ± 1.63 |
aAverage fold-induction of each lacZ reporter upon treatment with CaCl2 and CFW ± the standard error of the mean from two independent transformants assayed twice (n = 4). Statistically significant changes in the fold-induction in the mutant backgrounds relative to that observed in the wild type background are highlighted in bold