Figure 1.
Spray factor of LVS with different exposure chambers and nebulizers. F. tularensis strain LVS was grown in MHb overnight and then aerosolized into a whole-body or nose-only exposure chamber (A) or with a Collison nebulizer using a 1- or 3-jet nozzle into a nose-only exposure chamber (B). Aerosol samples were collected in an AGI and plated to determine aerosol concentration of LVS. Plots show the spray factor (ratio of aerosol concentration to nebulizer concentration) for individual aerosol exposures (circles and squares) and the median (line) value.