Table 2.

The MSP Primers Used in Our Study.

Gene	Nucleotide Sequence	PCR Product Size (bp)
Primer sequences for stage 1 PCR (methylation-independent)
p16	Forward: 5′-GAGGAAGAAAGAGGAGGGGTTG-3′	274
	Reverse: 5′-ACAAACCCTCTACCCACCTAAATC-3′
RASSF1A	Forward: 5′-GGAGGGAAGGAAGGGTAAGG-3′	260
	Reverse: 5′-CAACTCAATAAACTCAAACTCCC-3′
DAPK1	Forward: 5′-GGTTGTTTYGGAGTGTGAGGAGG-3′	236
	Reverse: 5′-CTAAAAACTCCCCCRATCCCT-3′
Stage 2 primer sequences.unmethylated allele-specific
p16	Forward: 5′-TTATTAGAGGGTGGGGTGGATTGT-3′	145
	Reverse: 5′-CAACCCCAAACCACAACCATAA-3′
RASSF1A	Forward: 5′-GGTTTTGTGAGAGTGTGTTTAG-3′	72
	Reverse: 5′-ACACTAACAAACACAAACCAAAC-3′
DAPK1	Forward: 5′-GGAGGATAGTTGGATTGAGTTAATGTTT-3′	80
	Reverse: 5′-CAAATCCCTCCCAAACACCAA-3′
Stage 2 primer sequences-methylated allele-specific
p16	Forward: 5′-GAGGGTGGGGCGGATCGC-3′	43
	Reverse: 5′-GACCCCGAACCGCGACCG-3′
RASSF1A	Forward: 5′-GGGGGTTTTGCGAGAGCGC-3′	203
	Reverse: 5′-CCCGATTAAACCCGTACTTCG-3′
DAPK1	Forward: 5′-ATAGTCGGATCGAGTTAACGTC-3′	152
	Reverse 5′-AAAACTAACCGAAACGACGACG-3′