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. 2012 Oct 1;5(5):327–334. doi: 10.1593/tlo.12163

Figure 1.

Figure 1

Mutation of tyrosine 845 to phenylalanine on EGFR inhibits cellular transformation. (A) MCF7 cells were induced to express wt-EGFR (WT) or Y845F-EGFR (Y845F) with 1 mg/ml doxycycline (Dox) in the presence or absence of EGF while plated in soft agar to measure transformation. Doxycycline was added every other day for 3 weeks of the transformation assay. (B) MCF7 cells were induced to express wt-EGFR or Y845F-EGFR with 1 mg/ml doxycycline for 48 hours and lysed. Lysates were immunoblotted using anti-EGFR and anti-β-actin antibodies. P values were calculated using Student's t test. EGF, *P = .0316, +P = .0064. Each experiment was performed in triplicate at least three times.