Figure 2.

Transient expression of Y845F-EGFR decreases cell proliferation. (A) SUM149 cells were transiently transfected with Y845F-EGFR on day 0, and cells were counted on days 1, 2, and 4. (B) Cell lysates were prepared from companion plates transfected with the growth assay and cultured for 4 days of the growth assays. Equal protein was separated by SDS-PAGE and was immunoblotted using EGFR, pY845-EGFR, pY1068-EGFR, pY1173-EGFR, and actin antibodies. Cell proliferation assays were performed in triplicate at least three times, and immunoblot analysis was performed at least three times. UT = pcDNA3 transfected.