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. Author manuscript; available in PMC: 2013 May 14.
Published in final edited form as: Biomacromolecules. 2012 Apr 27;13(5):1438–1447. doi: 10.1021/bm300150q

Table 3.

Properties of the peptide modified copolymers.

Peptide-
copolymer		 Derived
from
copolymer		 Copolymer
DPa
(−)		 Eq. of
peptide
for
thiolene
reactionb 		Allyl-MA
in
copolymer
(%)c 		Allyl
conversion
(%)d 		Peptide per
copolymer
(%)e 		Number of
peptides per
copolymerf
(−)
16 5 199 2.0 38 23.5 10.9 18
17 6 199 2.0 16 37.5 7.2 12
18 8 269 2.0 47 15.1 7.5 19
19 9 269 2.0 20 33.4 7.6 18
20 11 372 1.6 48 8.4 4.2 15
21 11 372 2.0 48 18.0 9.1 32
22 11 372 3.0 48 21.9 11.1 39
23 12 372 2.0 20 25.7 5.6 19
24 12 372 3.0 20 54.1 11.7 40
25 14 539* 2.0 47 11.9 6.0 30
26g 11 372 3.0 48 27.5 13.9 49
a

Number average degree of polymerization of the PPFMA precursor as determined from 1H-NMR spectroscopy from comparison of the protons on the RAFT agent end group to the -CH2 backbone peaks.

*

Note: the number average degree of polymerization of copolymer 14 is determined by DMF SEC.

b

Number of peptide equivalents with respect to allyl groups used during the thiol-ene reaction.

c

Determined with 1H-NMR spectroscopy.

d

Calculated from 1H-NMR spectra as: number of peptides/original number of allyl units.

e

Calculated from 1H-NMR spectra as: number of peptides per polymer /(total number of original allyl units prior to thiol-ene addition + number of HPMA units).

f

The number of peptide chains per copolymer based on the total polymer DP derived from 1H-NMR presented in Table 1.

g

Copolymer 26 functions as a negative control with the scrambled peptide grafted on to the copolymer via thiol-ene reaction.