Table 3.
Peptide- copolymer |
Derived from copolymer |
Copolymer DPa (−) |
Eq. of peptide for thiolene reactionb |
Allyl-MA in copolymer (%)c |
Allyl conversion (%)d |
Peptide per copolymer (%)e |
Number of peptides per copolymerf (−) |
---|---|---|---|---|---|---|---|
16 | 5 | 199 | 2.0 | 38 | 23.5 | 10.9 | 18 |
17 | 6 | 199 | 2.0 | 16 | 37.5 | 7.2 | 12 |
18 | 8 | 269 | 2.0 | 47 | 15.1 | 7.5 | 19 |
19 | 9 | 269 | 2.0 | 20 | 33.4 | 7.6 | 18 |
20 | 11 | 372 | 1.6 | 48 | 8.4 | 4.2 | 15 |
21 | 11 | 372 | 2.0 | 48 | 18.0 | 9.1 | 32 |
22 | 11 | 372 | 3.0 | 48 | 21.9 | 11.1 | 39 |
23 | 12 | 372 | 2.0 | 20 | 25.7 | 5.6 | 19 |
24 | 12 | 372 | 3.0 | 20 | 54.1 | 11.7 | 40 |
25 | 14 | 539* | 2.0 | 47 | 11.9 | 6.0 | 30 |
26g | 11 | 372 | 3.0 | 48 | 27.5 | 13.9 | 49 |
Number average degree of polymerization of the PPFMA precursor as determined from 1H-NMR spectroscopy from comparison of the protons on the RAFT agent end group to the -CH2 backbone peaks.
Note: the number average degree of polymerization of copolymer 14 is determined by DMF SEC.
Number of peptide equivalents with respect to allyl groups used during the thiol-ene reaction.
Determined with 1H-NMR spectroscopy.
Calculated from 1H-NMR spectra as: number of peptides/original number of allyl units.
Calculated from 1H-NMR spectra as: number of peptides per polymer /(total number of original allyl units prior to thiol-ene addition + number of HPMA units).
The number of peptide chains per copolymer based on the total polymer DP derived from 1H-NMR presented in Table 1.
Copolymer 26 functions as a negative control with the scrambled peptide grafted on to the copolymer via thiol-ene reaction.