Figure 4.

APRIL inducible knockdown. (a) Map of the inducible-lentiviral vector used to transduce CRC cell lines. (b) Representative images of LS180 cells transduced with an inducible shRNA vector treated with (right panel, I=induced) and without (left panel, NI=not induced) doxycycline. Pictures show overlay between red fluorescence (RFP) and brightfield microscopy. (c) Western blot analysis for APRIL expression in LS180 lines transduced with different shRNA targeting APRIL and cultured in the presence (I=induced) or absence (NI=not induced) of doxycycline. Constructs 17 and 18 show significant reduction of APRIL expression levels. β-actin is used as loading control