Fig. 1.

Determination of sensitivity of the nested-PCR for detecting Toxoplasma gondii B1 gene. Extracted T. gondii infected DNA was quantified, then serial dilutions were prepared, followed by the first and second round of PCR. Lanes 1 to 10 exhibits the 10⁰ to 10^-9 dilutions of infected DNA; lane C+ is positive control and lane C- is negative control. The results showed the sensitivity for the 1^st round of PCR was 17 pg/µl Toxoplasma gondii infected DNA (lane 6), and 170 fg/µl infected DNA (lane 8) for the nested-PCR. Lane M shows the 100 bp DNA size marker. Left black arrows show the size of PCR products for 1^st and 2^nd PCR products.