Figure 1.
1H-15N HSQC NMR titration experiments showing that myristylated and unmyristylated forms of HIV-1 MA do not interact with the protein-interactive domain of AP-3δ. (a)15N labeled myr(−)MA titrated with unlabeled AP-3δPID ; AP-3δPID: MA ratios: 0:1 (black), 0.5:1 (yellow), 1:1 (green), and 2:1 (red). (b) 15N labeled AP-3δPID titrated with unlabeled HIV-1 myr(−)MA; MA: AP-3δPID ratios: 0:1 (black), 0.5:1 (yellow), 1:1 (green), and 2:1 (red). (c,d) 15N-labeled myristylated MA titrated with AP-3δPID (natural isotopic abundance); AP-3δPID:MA ratios: 0:1 (black), 0.5:1 (yellow), 1:1 (green), and 2:1 (red). The expansion in (d) is provided to more clearly distinguish between the highly similar spectra.