Table 2.
Role of oxidoreductases in HNE metabolism in vascular smooth muscle cellsa
| Additives | Peak I (glutathione conjugates) |
Peak II (DHN) |
Peak III (HNA) |
Peak IV (HNE) |
Others |
|---|---|---|---|---|---|
| None (n=6) | 60±8 | 3±3 | 28±3 | 2±2 | 7±3 |
| Sorbinil (200 µM) (n=4) | 58±9 | 3±3 | 26±4 | 4±1 | 9± 4 |
| Tolrestat (10 µM) (n=3) | 58±5 | 4±1 | 25±3 | 4±2 | 9±5 |
| 4-MP (400 µM) (n=3) | 56±3 | 4±1 | 28±2 | 5±2 | 7±2 |
| Cyanamide (2 mM) (n=3) | 76±8* | 4±2 | 10±3* | 3±3 | 7±1 |
a
VSMC were incubated with 50 nmol [3H]-HNE and indicated additives for 30 min at 37°C. At the end of the incubation, the media were collected and separated by HPLC as described in the text.
*
P<0.05 compared with VSMC incubated with [3H]-HNE without any additives.