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. Author manuscript; available in PMC: 2012 Oct 11.
Published in final edited form as: Biochem J. 2010 Dec 15;432(3):557–563. doi: 10.1042/BJ20100557

Figure 4. Quantification of translocation and maturation efficacy of PC-PLC propeptide mutants.

Figure 4

The strain numbers are indicated on the left. The propeptide sequence with the corresponding amino acid sequence numbers are listed above the schematic diagram representing each mutant. The broken line indicates that the corresponding amino acid residue was deleted and solid lines indicate propeptide sequences present within a mutant. Letters indicate the amino acids that were used for substitution mutants. The ratios were calculated in reference to the behaviour of wild-type PC-PLC expressed in HEL-1031 (the ratio for wild-type PC-PLC = 1). Wild-type PC-PLC was translocated with an efficacy of 16.4 ± 3.0% (means ± S.E.M. for 14 experiments). The efficacy of maturation of wild-type PC-PLC was 87.6 ± 0.8% (means ± S.E.M. for 15 experiments). (n) represents the number of immunoprecipitation experiments performed with each strain. NA, not applicable (the proform and mature form of PC-PLC could not be resolved by SDS/PAGE); ND, not determined. Statistical analysis of data was performed as described in the Experimental section. *P < 0.01.