Table 1.
Mobile phase | Retention Capacity (k/) | Theoretical plates (N) | Tailing Factor (T) | Resolution (R) | Peak shape |
---|---|---|---|---|---|
Methanol: Phosphate Buffer | |||||
pH 2.8 (62:38) | |||||
Moxifloxacin |
|
4521 |
1.17 |
|
+++ |
Prednisolone |
0.92 |
6568 |
1.01 |
13.66 |
+++ |
Methanol: Phosphate Buffer | |||||
pH 3.5 (62:38) | |||||
Moxifloxacin |
|
4251 |
1.29 |
|
+++ |
Prednisolone |
0.84 |
5784 |
1.18 |
12.24 |
+++ |
Methanol: Phosphate Buffer | |||||
pH 4.5 (62:38) | |||||
Moxifloxacin |
|
4122 |
1.47 |
|
+++ |
Prednisolone |
0.54 |
5541 |
1.14 |
9.45 |
--- |
Methanol: Phosphate Buffer | |||||
pH 6.5 (62:38) | |||||
Moxifloxacin |
|
3801 |
1.40 |
|
+++ |
Prednisolone | 0.41 | 4787 | 1.28 | 11.87 | --- |
Chromatographic conditions, mobile phase methanol: 0.018 M phosphate buffer 62:38, pH 2.8, or 3.5, or 4.5, or 6.5, Column BDS Hypersil C8 (250 X 4.6, 5 μm), flow rate 1.5 mL min-1,
injection volume 20 μL, wavelength 254 nm, +++ is peak acceptable and --- is peak not acceptable.