Table 3.
The number of CD-epitope cores in γ-gliadin transcripts ofT. aestivum
|
epitope |
Gli-A1 |
Gli-A1 |
Gli-B1 |
Gli-B1 |
Gli-B1 |
Gli-D1 |
Gli-D1 |
∑ |
|---|---|---|---|---|---|---|---|---|
| Group 6 | Group 7 | Group 3 | Group 5 | Group 8 | Group 9 | Group 10 | ||
| DQ2-γ-VIIb3* |
701 |
144 |
72 |
230 |
57 |
1257 |
50 |
2511 |
| DQ2-γ-VI3 |
224 |
0 |
0 |
0 |
38 |
678 |
50 |
990 |
| DQ2-glia-γ2a6 |
140 |
30 |
36 |
59 |
38 |
684 |
0 |
987 |
| DQ2-γ-II(Glia γ30)3,4 |
136 |
0 |
41 |
125 |
0 |
293 |
61 |
656 |
| DQ2-γ-I (Glia-γ1)2 |
120 |
38 |
41 |
115 |
19 |
293 |
61 |
567 |
| DQ2-γ-VIIa3 |
0 |
0 |
0 |
115 |
19 |
0 |
0 |
134 |
| DQ2-γ-IV5 |
0 |
0 |
0 |
0 |
0 |
0 |
61 |
61 |
| DQ2-γ-III5* |
0 |
0 |
0 |
0 |
0 |
0 |
50 |
50 |
| DQ2-glia-γ2b6 |
0 |
0 |
0 |
0 |
0 |
0 |
50 |
50 |
| Nepitopes |
1321 |
212 |
190 |
644 |
171 |
3205 |
383 |
6006 |
| Ntranscripts |
140 |
38 |
41 |
125 |
19 |
293 |
61 |
717 |
| Nepitopes/Ntranscripts |
9.4 |
5.6 |
4.6 |
5.2 |
9.0 |
10.9 |
6.3 |
8.4 |
| Nepitopes/Ntranscripts | 8.6 (1533/178) | 5.4 (1005/185) | 10.1 (3588/354) | |||||
The number of T-cell stimulating sequences involved in CD (9 mer epitope cores) found in a set of 717 γ-gliadin transcripts from T. aestivum (genome ABD) attributed to respectively Gli-A1 (topology group 6 and 7), Gli-B1 (topology group 3, 5, and 8) and Gli-D1 (topology group 9 and10). The sequence topology was obtained by Neighbor joining analysis (see Figure 2). The frequency of T-cell epitopes in γ- gliadin transcripts (Nepitopes/Ntranscripts) is calculated.