Table 1.
Effect of factor treatment on the mean number of neurons, and the neuron to glial cell ratio in cultures of enteric ganglion cells after 12 and 36 hours incubation
| |
Control |
NT-3 |
GDNF |
|||
|---|---|---|---|---|---|---|
| Neurons | Neurons/ glia | Neurons | Neurons/ glia | Neurons | Neurons/ glia | |
| 12 hours |
159 (±0.36) |
0.31 |
169 (±0.41) |
0.36 |
176 (±0.55) |
0.37 |
| 36 hours | 119 (±0.32) | 0.23 | 151 (±0.37) | 0.29 | 195 (±0.44) | 0.34 |
Cultures established as described in the Methods were grown in the presence of NT-3 or GDNF, both at 10 ng/ml concentration, fixed after 12 or 36 hours and subsequently stained with PGP 9.5. Values represent the mean of neuronal numbers counted in 3 separate experiments, each treatment consisting of 3 replicas per experiment. Values in parentheses represent ± SEM.