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. 2012 Oct 11;8(10):e1003006. doi: 10.1371/journal.pgen.1003006

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© 2012 Coléno-Costes et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Squash of salivary gland polytene chromosomes from an esg::Gal4>UAS::FH-cortoCD-231 third instar larva. Anti-FLAG immunostaining shows that FH-CortoCD binds chromatin at many sites (left: DAPI, middle: anti-FLAG, right: merge). Arrow-heads point to DAPI-free Corto bands. (B) Magnifications of the tip of polytene chromosome 3L (bands 61 to 63) from third instar larvae, either esg::Gal4>UAS::FH-cortoCD immunostained with anti-FLAG antibody or w¹¹¹⁸ immunostained with anti-Corto antibody (DAPI, immunostaining, merge). Bottom: conformity between endogenous Corto and FH-CortoCD binding sites.