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. 2012 Oct 11;8(10):e1002995. doi: 10.1371/journal.pgen.1002995

Figure 1. SUVR5 zinc finger domain binds specific sequences of DNA that map to the promoters of genes.

Figure 1

a, Domain structure of SUVR5 (Poly-Asp: domain of unknown function rich in Asp residues); b, enriched motifs identified in the sequencing data obtained from the SELEX experiments; c, meta-gene analysis of the genomic SELEX (gSELEX) reads showing preferential binding of the SUVR5 zinc finger domain to the 3 Kb region upstream of protein coding genes (PCG). The results obtained after exponential selection of the binding sites for 9 cycles are shown (×9) in contrast with the results obtained after only one cycle of enrichment (×1), included as control; d, mobility shift assay with increasing amounts of GST-zinc finger domain (100, 250 and 500 ng) added to a binding reaction with either an unspecific oligonucleotide probe or a specific probe including the identified binding motif sequence.