Skip to main content
. 2012 Oct 11;7(10):e47638. doi: 10.1371/journal.pone.0047638

Figure 1. Plasminogen is recruited by Sbi and Efb.

Figure 1

(A) Plasminogen bound to Sbi and Efb (lane 1 and 2). The accuracy of the recombinant staphylococcal proteins was confirmed as both, Sbi and Efb, bound to C3 (lane 3 and 4) and Sbi also bound IgG. Recombinant Sbi or Efb was separated by SDS-PAGE and either blotted to a membrane (lane 1–6) or silver stained (lane 7–8). Membranes were incubated with biotinylated PLG (PLGb), C3, or HRP- coupled IgG and bound proteins were detected as indicated. (B) Binding of plasminogen to Sbi or Efb was confirmed by ELISA. Bacterial proteins (equimolar) were immobilized and PLGb was applied and detected using streptavidin-HRP. Plasminogen binding to Sbi and Efb was comparable to plasminogen binding to the borrelial protein CRASP-5. Plasminogen did not bind to HSA or the plate (buffer). Data represent mean values ± standard deviations from three independent experiments. (C) Plasminogen was recruited to Sbi and Efb. Non-labeled plasminogen was applied to immobilized bacterial proteins. Bound proteins were eluted, separated by SDS-PAGE and plasminogen (92 kDa) was detected by Western blot analysis. CRASP-5 bound plasminogen in contrast to HSA and buffer. A representative experiment out of three is shown.