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. 2012 Oct 11;7(10):e47638. doi: 10.1371/journal.pone.0047638

Figure 4. Plasmin complexed with C3(b) and Sbi or Efb degrades C3 and C3b within the complex.

Figure 4

Plasmin degraded C3/C3b within the Sbi:plasmin:C3/C3b or Efb:plasmin:C3/C3b complexes (lane 1 and 4) and C3/C3b cleavage products appeared (marked with arrows). Bacterial proteins were immobilized (equimolar) and plasminogen was added together with C3 (A) or C3b (B). The activator SAK was applied and C3/C3b cleavage was followed by Western blot analysis using anti-C3-HRP (Fab). The mobility of the α (α ´) and β chains of C3/C3b and the cleavage products are indicated by arrows. CRASP-5 and HSA had no effect on cleavage (lane 7 and 8). Data shown are representative of three independent experiments. (C) Proposed schematic model of the C3 cleavage products generated by plasmin modified after [25], [32]. The C3 dg cleavage product is not recognized by the C3-antibodies used in this study.