Figure 2. FKHRL1/TM induces cytotoxicity, caspase pathway activation, and apoptosis. A2058 and DM6 melanoma cell lines were infected at increasing doses (0–100 MOI) of either Ad-LacZ or Ad-FKHRL1/TM. At 72 h cells were analyzed by MTT, and western blot assays. (A) MTT assay was used to determine cell survival comparing Ad-LacZ with Ad-FKHRL1/TM treatments. Each point represents the mean ± SD of three independent experiments (p < 0.05). (B) Expression of FKHRL1/TM, proenzyme CPP32/caspase-3 (CPP32), PARP and cleaved components of PARP (PARP/C) were detected by western blot. α-Actin was used to demonstrate equal loading for each lane. (C) A2058 and DM6 melanoma cell lines were not infected (Mock) or infected with either Ad-LacZ or Ad-FKHRL1/TM at an MOI of 100. Three days post-infection the percentages of apoptosis were determined by annexin V staining, and analysis by FACScan flow cytometer with FlowJo software. Similar results were obtained in three independent experiments. A representative experiment is shown.