Figure 4. Inhibition of Fas-L-mediated cell death. (A) DM6 or A2058 cells were cultured with increasing concentrations of Fas/Fas-L antagonist Kp7-6 followed by infection with either Ad-LacZ or Ad-FKHRL1/TM at an MOI of 50. At 72 h an MTT assay was performed to determine cell survival. Ad-FKHRL1/TM alone was compared with Ad-FKHRL1/TM in presence of Kp7-6. Each point represents the mean ± SD of three independent experiments (*p < 0.05). (B) A2058 cells were cultured in absence or presence of Fas/Fas-L antagonist Kp7-6 at 10 µg/mL followed by mock infection or infection with either Ad-LacZ or Ad-FKHRL1/TM at an MOI of 100. Western blot and bar graphs of procaspase-8 expression after adenovirus infection. Bars represent mean ± SEM expressed as percentage of change from 3 separate experiments, (*p < 0.05) decrease in the level of expression. α-Actin was used to demonstrate equal loading for each lane. FKHRL1/TM expression was also confirmed.