Figure 5. FKHRL1/TM sensitizes melanoma cells to CH-11 mediated-apoptosis and downregulates IAP family members. (A) A2058 or DM6 cells were infected with either Ad-LacZ or Ad-FKHRL1/TM at an MOI of 10 +/− treatment with CH-11 at 1 µg/mL. Annexin V staining was used to determine the percentage of apoptosis at 72 h after treatment by FACScan flow cytometer with FlowJo software. Ad-FKHRL1/TM infection alone was compared with Ad-FKHRL1/TM in combination with CH-11. Each bar represents the mean ± SD of three independent experiments (*p < 0.05). (B) A2058 or DM6 cells were no infected (mock) or infected with either Ad-LacZ or Ad-FKHRL1/TM at an MOI of 100 at 72 h post-infection expression of X-IAP, C-IAP-1, or C-IAP-2 were analyzed, a representative experiment is shown from three performed.