Figure 4. DMTC causes the accumulation of cells with abnormal nuclei and triggers apoptosis in pancreatic cancer cells. (A) Immunofluorescence images of microtubules (red) and DNA (blue) in EPP85 cells treated for 24 h with 20 nM DMTC or the DMSO control. (B) Cells were treated with 20 nM DMTC or DMSO (control) for 0, 24 or 48 h, and the percentage of cells with abnormal nuclei was then quantified by immunofluorescence staining of microtubules and DNA as in (A). Values, averages of three independent experiments; bars, SD; **p < 0.01. (C) Quantitation of apoptosis by annexin V/PI staining in EPP85 cells treated for 72 h with 20 nM DMTC or DMSO (control).