Skip to main content
. 2012 Oct 11;7(10):e47091. doi: 10.1371/journal.pone.0047091

Figure 1. S. pombe swi7-1 mutant is sensitive to DNA damaging agents.

Figure 1

A. Drop-assay analysis. Drops containing approximately 100 cells from mutant and wild type strains were placed on medium, containing methyl methanesulfonate (MMS) or hydrohyurea (HU) at concentrations given, or were exposed to UV at the given intensities. The plates were incubated at 33° until colonies were formed. B. Survival of yeast cells exposed to MMS or HU. Logarithmically growing cultures were treated with either 0.03% MMS or 12 mM of HU. Samples were taken at indicated time points, and dilution series of cells for each culture were plated on YEA media and incubated at 33°C until colonies were formed. Strains shown in panel A and B are wt (JZ60), swi7-1 (JZ468), chk1 (JZ473), rad3 (JZ474), cds1 (JZ475), swi1 (E111), swi3 (E146).